Research on the rapid selection of suitable solvent system for HSCCC based on the HPLC polarity parameter model

Photo by rawpixel on Unsplash

Advisor: Jintian Tang; Professor in Department of Engineering Physics, Tsinghua University; Director of the Key Laboratory of Particle & Radiation Imaging, Ministry of Education.

Project Area: Analytical Chemistry - Chromatography.

- This project provided a rapid and simple method for the separation of unknow compounds. I used liquid-liquid separation technology coupled with High-Speed Countercurrent Chromatography (HSCCC) to separate high-purity compounds from complex natural compounds.

- Found and experimentally verified a strong relationship between the compound polarity calculated through HPLC and the average polarity of the 2-liquid-phase solvent system in which the compound was evenly distributed. I established, refined and verified the parameter model through experiments. Based on this model, the goal of quickly finding the applicable HSCCC two-phase solvent system based on the compound polarity model was achieved.

- Mastered HPLC, HSCCC, other chromatographic techniques, and basic analytical chemistry experimental skills after finishing this project. First-authored paper has been submitted to Journal of Chromatography A and is under review.

Xiaohan Zhu
Xiaohan Zhu
Graduate Student

Graduate from Tsinghua Univ TEEP. Keen interest in Bio+